RNA

Part:BBa_K3001010:Design

Designed by: Sydnee Calhoun   Group: iGEM19_Lethbridge_HS   (2019-10-10)


Lbu crRNA targeting GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A direct repeat stem loop is added to enable the interaction between the crRNA and Cas13a Lbu. The target sequence was selected since it is specific for GFP to minimize the potential for off-target effects.


Source

Designed by Laura Keffer-Wilkes based on designs by Munich 2017. Synthesized by IDT.

References